Generator
P43-gsiB

Part:BBa_K143050

Designed by: Chris Hirst   Group: iGEM08_Imperial_College   (2008-09-29)

Promoter 43 and RBS gsiB for B. subtilis


Constitutive promoter 43(BBa_K143013) coupled to the strong Ribosome Binding Site gsiB(BBa_K143020) from B. subtilis.


P43-gsiB can be used in the context of a Ribosomes per second (RiPS) output generator.


To get the highest level of translation from this Promoter-RBS combination it must be connected to a coding region preceded by a coding region prefix1. A standard prefix will increase the distance between the RBS and the start codon, reducing translational efficiency.


Sequence and Features



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

<biblio>

  1. 1 https://parts.igem.org/Assembly:RBS-CDS_issues

</biblio>


[edit]
Categories
//chassis/prokaryote/Bsubtilis
Parameters
None